Ovarian Function/Dysfunction
Session: Poster Session A
dong Mok Lee
59,Yangho-gil, Yeoungchean-si, Gyeongsangbuk-do, 338822,Rep, of korea
Korea Institute of Industrial Technology
Yeongchean-si, Kyongsang-bukto, Republic of Korea
Dong-Mok Lee1; Byoung Boo Seo2; Min Gwon Kim1
1. Korea Institute on Industrial Technology, Yeongcheon-si Gyeongsangbuk-do, 38822 Republic of Korea
2. Department of Natural Resources, Daegu University, Gyeongsan-si, Gyeongbuk-do, 38453, Republic of Korea
Animal cell culture is a highly complex process in which cells grow under specific conditions, necessitating various techniques such as additives and coatings to enhance culture efficiency. Among these, collagen coating is an effective method for improving cell adhesion and proliferation. However, using a single substance alone presents challenges in replicating the complex environment of living tissues. To address this limitation, this study investigated the effects of utilizing decellularized extracellular matrix (dECM) derived from biomaterials as a coating material in cell culture.
The dECM used in this study was extracted from porcine ovaries through a decellularization process. The extracted dECM was sterilized using electron beam irradiation, and its components, including deoxyribonucleic acid (DNA), collagen, and glycosaminoglycans (GAGs), were analyzed. The dECM was then coated onto culture plates, and its presence was confirmed using a scanning electron microscope (SEM). To evaluate its impact, C2C12 cells were cultured on dECM-coated plates, and adhesion and proliferation experiments were conducted at different concentrations. Additionally, metabolic products in the culture medium and cellular gene expression were analyzed.
In this study, an optimized decellularization process was established, which effectively removed 99% of DNA while maintaining the collagen and GAG content. Electron beam sterilization at a 1 kGy irradiation dose further reduced the DNA content by 46%, while preserving 93% of collagen and 92% of GAGs, confirming the effectiveness of this approach. In cell culture experiments, dECM coating enhanced cell adhesion and proliferation, similar to collagen coating. Notably, at a dECM concentration of 0.01%, cell proliferation exceeded that observed with collagen coating(p< 0.01).
In conclusion, porcine ovarian dECM was demonstrated to effectively support cell adhesion and proliferation, comparable to collagen. These findings suggest that dECM can serve as a viable alternative to collagen and may be a valuable biomaterial for cell culture and basic research applications.