(195) Elevated Levels of Circulating Extracellular Vesicles Inhibit Angiogenesis in Severe Early-Onset Preeclampsia

Scout Bowman-Gibson¹; Traci Rackett²; Tracy Attikple²; Hannah DeRespiris²; Josie Lowell²; Katherine Wilcher²; Rose Maxwell²; David Dhanraj²; Thomas L. Brown^1,2

1: Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of Medicine, Wright State University, Dayton, United States

2: Department of Obstetrics and Gynecology/Premier Health, Boonshoft School of Medicine, Wright State University, Dayton, United States

Abstract Text: Preeclampsia is a common pregnancy-associated disorder and a leading cause of maternal and fetal morbidity and mortality. Circulating levels of maternal extracellular vesicles (EVs) are significantly elevated in patients with severe early-onset preeclampsia (sEOPE); however, it is not known how EVs contribute to the pathophysiology of this condition. To address this question, we evaluated the functional activity of EVs on cell migration and capillary-like tube formation in human umbilical vein endothelial cells. Our results indicate that plasma-derived maternal EVs from sEOPE patients significantly inhibits cell migration and angiogenesis, compared to normotensive controls, and suggests that EVs may be an important mediator in the pathogenesis of this disorder.